Key Proteins of Activating Cell Death Can Be Predicted through a Kainic Acid-Induced Excitotoxic Stress

Table 1

Summary of five differentially regulated proteins in kainic acid-induced apoptosis, compared to KA-induced necrosis, as detected by two-dimensional differential in-gel electrophoresis (DIGE) and mass spectrometry.

Spot number	Protein	Accession (ID) number^a	MW (Da)^b/PI	Change^c	Sequence Coverage (%)^d	Mowse score^e
1	3-Mercaptopyruvate sulfurtransferase	NP_620198.1	32809/5.88	1.6	13%	67
2	Pyruvate dehydrogenase beta	NP_001007621.1	35842/6.20	1.7	27%	185
3	Tubulin beta 5	NP_035785.1	49639/4.78	1.8	9%	112
4	Heat shock protein 60 (HSP60)	CAA37654.1	57890/5.35	2.2	19%	252
5	Guanine nucleotide binding protein	NP_001013932.1	37339/5.74	1.2	16%	122

Significantly upregulated proteins demonstrated a greater than 1.5-fold increase in expression.
^aReference for protein identification from the protein database at the National Center for Biotechnical Information (NCBI).
^bMW: molecular weight.
^cIntensity of spots from two samples run in the 2D-DIGE gel as measured by IMAGEMASTER 2D software.
^dSequence coverage (%) indicates the number of amino acids spanned by the assigned peptides divided by the sequence length.
^eMowse score indicates the probability that the match between the database and a spectrum is a random event.