Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 1

DISC1 and DBZ mRNA is expressed in oligodendrocytes in the corpus callosum (CC) of the mouse brain. (a) Real-time PCR analysis of DISC1 in CC at the indicated stages. Data are expressed from at least three independent experiments. DISC1 mRNA levels at P7 were higher than at later time points. , as assessed via a one-way ANOVA followed by Tukey’s test. (b) Primary cultured rat OPCs were harvested at indicated times after platelet-derived growth (PDGF) deprivation. mRNA was quantified by qRT-PCR. Data are expressed from at least three independent experiments. DISC1 mRNA levels at 0 h were higher than at later time points. , as assessed via a one-way ANOVA followed by Tukey’s test. (c) Postnatal development of DBZ mRNA in the CC of mice from P0, P7, P14, P21, and adults. DBZ mRNA-expressing cells were first identified at P7. The number and intensity of the reaction of DBZ-positive cells reached to peak at P14, followed by its progressive reduction until adulthood. Scale bar: 50 μm. (d) Quantification of the proportion of DBZ+/PDGFRα+ and DBZ+/CCI+ cells. Double labeling with in situ hybridization and immunohistochemical analysis of brain sections from P14 mice with the antisense RNA probe to DBZ and antibodies against Olig2, PDGFRα, and CC1, respectively. (e) Western blot analysis of DBZ and MBP in the CC at the indicated stages. DBZ protein expression peaked at P14 before MBP expression. (b) is adapted with permission from Hattori et al. [20] and (c)–(e) are adapted with permission from Shimizu et al. [19].

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