Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 13

Repeated exposure to WIRS upregulates NDRG1 phosphorylation in the fiber tracts via SGK1 activation. (a) In situ hybridization images of Ndrg1 mRNA. Dark-field photomicrographs show the distribution of Ndrg1 mRNA-expressing cells in the mouse brain on the sagittal plane. Sections were hybridized with ³⁵S-labeled antisense RNA probe for Ndrg1 mRNA. As controls, adjacent sections were hybridized with ³⁵S-labeled sense RNA probe (inset). Scale bar = 5 mm. (b) Immunoprecipitation and western blot analysis show that repeated exposure to WIRS elevated the interaction between SGK1 and NDRG1 (second column). However, NDRG1 expression did not increase in the CC (first column). (c) Western blot analysis shows that repeated exposure to WIRS elevated phosphorylated NDRG1 levels in the CC. (a)–(c) are adapted with permission from Miyata et al. [37].

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