Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 14

Repeated exposure to WIRS upregulates adhesion molecules expression levels in oligodendrocytes of the fiber tracts. (a) Immunoprecipitation and western blot analysis show that repeated exposure to WIRS elevated the interaction between NDRG1 and β-catenin (second panel) and that the expression levels of β-catenin, N-cadherin, and α-catenin were elevated in the corpus callosum. (b) Immunohistochemical analysis of β-catenin in the CC demonstrates increased labeling of the processes of the oligodendrocytes (i.e., greater number and intensity) in mice exposed to repeated WIRS. Scale bar = 50 μm. (c) Immunocytochemical analysis of β-catenin in SK-N-SH cells overexpressing nonphosphorylated (NP-NDRG1) or constitutively phosphorylated NDRG1 (CP-NDRG1). Increased expression of β-catenin due to the overexpression of the CP-NDRG1 was mostly observed on the surfaces of the cell bodies and the processes of SK-N-SH cells. Scale bar = 20 μm. (a)–(c) are adapted with permission from Miyata et al. [37].

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