Disturbance of Oligodendrocyte Function Plays a Key Role in the Pathogenesis of Schizophrenia and Major Depressive Disorder

Figure 3

DISC1 knockdown promotes oligodendrocyte differentiation. (a) Primary cultured rat OPCs transfected with control-siRNA, DISC1-siRNA-1, or DISC1-siRNA-2 were lysed 72 hours after siRNA transfection and analyzed by western blotting. (b, d) Primary cultured rat OPCs were transfected with control-siRNA, DISC1-siRNA-1, or DISC1-siRNA-2 and cultured in medium containing PDGF for 72 hours then fixed for immunostaining. Primary cultured rat OPCs were immunostained with anti-CNPase-antibody (b) or anti-β-tubulin antibody (d) and analyzed as described here. versus control-siRNA. Scale bars = 50 μm. (c) DISC1 knockdown mediated increase of CNPase mRNA was rescued by overexpression of DISC1. Primary cultured rat OPCs were infected with GFP- or DISC1-GFP-Adv 24 hours after control- or DISC1-siRNA transfection. Forty-eight hours after the infection, mRNA level of CNPase was quantified by qRT-PCR. Data are expressed as mean ± SEM of at least three independent experiments. versus control-siRNA and GFP-Adv. (a)–(d) are adapted with permission from Hattori et al. [20].

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