Research Article

Nampt/PBEF/Visfatin Upregulation in Colorectal Tumors, Mirrored in Normal Tissue and Whole Blood of Colorectal Cancer Patients, Is Associated with Metastasis, Hypoxia, IL1β, and Anemia

Figure 4

Effect of exogenous visfatin on growth of colon adenocarcinoma cell line SW620. Cells were grown with 10% FBS to 80% confluence and subsequently medium had been changed on fresh serum-supplemented or serum-free medium with 0, 20, 100, and 500 ng/mL recombinant visfatin, in which cells were grown for 8 or 24 hours. Results are presented as relative growth of stimulated versus unstimulated (100%) cells, determined with SRB assay.