Research Article
Interleukin-1
β
Modulates Melatonin Secretion in Ovine Pineal Gland: Ex Vivo Study
Table 1
All genes analyzed by real-time PCR are listed with their full name and abbreviation.
| | GenBank Acc. number | Gene | Amplicon size [bp]
| Forward/reverse | Sequence |
| | NM_001034034 | GAPDH: glyceraldehyde-3-phosphate dehydrogenase |
134 | Forward | AGAAGGCTGGGGCTCACT | | Reverse | GGCATTGCTGACAATCTTGA |
| | U39357 | ACTB: beta-actin |
168 | Forward | CTTCCTTCCTGGGCATGG | | Reverse | GGGCAGTGATCTCTTTCTGC |
| | NM_001076910 | PPIC: cyclophilin C |
131 | Forward | ACGGCCAAGGTCTTCTTTG | | Reverse | TATCCTTTCTCTCCCGTTGC |
| | BC108088.1 | HDAC1: histone deacetylase 1 |
115 | Forward | CTGGGGACCTACGGGATATT | | Reverse | GACATGACCGGCTTGAAAAT |
| | NM_001009461 | AANAT: arylalkylamine-N-acetyltransferase |
154 | Forward | CGAGAGGCCTTCATCTCTGT | | Reverse | GTCTCTCCTCATCCCACAGG |
| | KC290950 | HIOMT: hydroxyindole-O-methyltransferase |
167 | Forward | AGCTTCCATGAAGGGGATTT | | Reverse | AGGAGGCTCTCGATGACCAG |
| | X54796.1 | IL-1: interleukin-1 beta |
137 | Forward | CAGCCGTGCAGTCAGTAAAA | | Reverse | GAAGCTCATGCAGAACACCA |
| | NM_001206735.1 | IL-1R1: interleukin 1 receptor, type I |
124 | Forward | GGGAAGGGTCCACCTGTAAC | | Reverse | ACAATGCTTTCCCCAACGTA |
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