Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris

Figure 1

Gel electrophoresis of PCR products of three randomly selected P. pastoris GS115 transformant amplified using XbaI (forward) and PmlI (reverse) primers. M, 1 kb DNA ladder; Lanes 1, 3, and 4 are PCR amplicons of GS115/pPICZαB/α-amylase transformants. Lane 2 has no band represents pPICZαB expression vector.