Expression and Characterization of <i>Geobacillus stearothermophilus</i> SR74 Recombinant <i>α</i>-Amylase in <i>Pichia pastoris</i>

<div>(a) SDS-PAGE (12%) analysis of purified <i>α</i>-amylase. M, unstained protein molecular weight marker; Lane 1, purified <i>α</i>-amylase SR74; Lane 2, crude extract of expressed <i>α</i>-amylase SR74. (b) Western blot analysis of recombinant <i>α</i>-amylase. M, unstained protein molecular weight marker; Lane 1, crude extract of <i>α</i>-amylase. Both arrows (<svg height="6.44133pt" id="M4" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -6.39151 13.0179 6.44133" width="13.0179pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M918 236V289H166L271 439L245 456C169 372 95 299 55 263C95 227 169 154 245 70L271 87L167 236H918Z" id="g54-147"></path><glyph.data ascent="3443" descent="-2856" horiz-adv-x="990" vert-adv-y="990"></glyph.data></g></svg>) indicate the molecular weight of <i>α</i>-amylase at 59 kDa.</div>

BioMed Research International

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Figure 2

Figure 2: Expression and Characterization of <i>Geobacillus stearothermophilus</i> SR74 Recombinant <i>α</i>-Amylase in <i>Pichia pastoris</i> 

BioMed Research International

Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris

Figure 2