Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris

Figure 3

(a) Effect of various media on α-amylase SR74 activity at 48 h of cultivation of recombinant P. pastoris GS115 when induced with 0.5% (v/v) of methanol. Data are presented as ±SD of triplicates. (b) Effect of different methanol concentrations on α-amylase SR74 production by P. pastoris GS115 in 48 h cultivation in YPTM media. Data are presented as ±SD of triplicates. (c) Activity profile of recombinant α-amylase SR74 colony 21 of P. pastoris. Data are presented as ±SD of triplicates.

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