Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris

Table 1

PCR conditions.

PCR conditions	Temperature (°C)	Time (min)
Initial denaturation	94	4
Denaturation	95	1	30 cycles
Annealing	65	1
Extension	72	1
Final extension	72	7
Pause	4	0