Research Article
Expression and Characterization of Geobacillus stearothermophilus SR74 Recombinant α-Amylase in Pichia pastoris
Table 2
Summary of the purification of His-tagged recombinant α-amylase.
| | Purification step | Total activity (U) | Total protein (mg) | Specific activity (U mg−1) | Recovery (%) | Purification (fold) |
| | Crude | 508 | 6.4 | 79.4 | 100 | 1 | | Ni-Sepharose | 267.2 | 1.8 | 151.8 | 52.6 | 1.9 |
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