Effect of Luteolin and Apigenin on the Expression of Oct-4, Sox2, and c-Myc in Dental Pulp Cells with In Vitro Culture

Figure 1

Effect of luteolin and apigenin on the expression of Oct-4, Sox2, and c-Myc in DPCs. Real-time PCR showed that mRNA expression of Oct-4 ((A1), (A4)), Sox2 ((A2), (A5)), and c-Myc ((A3), (A6)) in DPCs with luteolin ((A1)–(A3)) and apigenin ((A4)–(A6)) treatment increased at a time- and dose-dependent pattern. Expression of Oct-4 (A1), Sox2 (A2), and c-Myc (A3) was significantly upregulated in DPCs after being treated with luteolin at the concentrations of 1, 5, and 10 μmol/L at 5 d (, ), albeit they did not show any difference at 0 and 3 d . After 3 d treatment, expression of Oct-4 (A4) was significantly upregulated in DPCs with apigenin induction at the concentrations of 1, 5, and 10 μmol/L, Sox2 (A5) was significantly upregulated in DPCs with apigenin induction at the concentrations of 1, 5 μmol/L, and c-Myc (A6) was significantly upregulated in DPCs with apigenin induction at the concentration of 5 μmol/L (, ). After 5 d treatment, expression of Oct-4 (A4), Sox2 (A5), and c-Myc (A6) was significantly upregulated in DPCs with apigenin induction at the concentration of 10 μmol/L (,). The result of real-time PCR indicated that the expression of Oct-4 (C1), Sox2 (C2), and c-Myc (C3) was significantly upregulated in DPCs at both passages 3 and 7 with luteolin and apigenin induction (, ). The protein expression of Oct-4 ((B1), (B4)), Sox2 ((B2), (B5)), and c-Myc ((B3), (B6)) was significantly upregulated in DPCs at passages 3 and 7 with luteolin induction (, ), which was similar to the expression pattern in DPCs at passage 7 with apigenin induction (, ). Whereas in DPCs, at passage 3 with apigenin induction, only Sox2 showed significant upregulation (), Oct-4 and c-Myc did not show any significant difference with the control group ().

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