Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns

Table 1

List of primers and sequences.

Methods	Gene name	Primer name	Nucleotide sequence (5′-3′)
Reverse transcription		Oligo(dT)18	TTTTTTTTTTTTTTTTTT
Partial sequence	IFNGR1	F1	TTAAAGCTGTTGTTGGATCA
		R1	CAATCACASGYTGTTCTTC
	IFNGR2	F2	CTGAGGTGGTCTCCTGTTA
		R2	TCAAATACTCTTCAAWGTGTG
3RACE		AP	CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGC (T)18
		AP1	CCAGTGAGCAGAGTGACG
		AP2	GAGGACTCGAGCTCAAGC
5RACE		AAP	GGCCACGCGTCGACTACGGGIIGGGIIGGGIIGGGIIG
		AUAP	GGCCACGCGTCGACTAGTAC
3RACE-GSP	IFNGR1	G1-3GSP1	GGCACCAGACAAAGTGGAAGAGTC
		G1-3GSP2	TGCAGAAGATTACAGAGGAGGTCC
	IFNGR2	G2-3GSP1	TGGACTGCGGAGAATCCCGGAATG
		G2-3GSP2	CAATGAGTGAGACAACCAGAGCTG
5RACE-GSP	IFNGR1	G1-5GSP1	ATCCCAAAAAGTCACC
		G1-5GSP2	GAAATACAGGATGGTAAATATCAAC
		G1-5GSP3	GAGAGATCCAGTTTTGGAGGTC
	IFNGR2	G2-5GSP1	CATTCTCCCAGTAG
		G2-5GSP2	AGTCACGCTGTTCACTTTAGGG
		G2-5GSP3	ATTCCACCCAGTCAGAAGTCAT
Real-time PCR	IFNGR1	goqRT-G1-F	GCATTCAGGTTCCTCTTG
		goqRT-G1-R	AAGCGTTATCCATGTTCAG
	IFNGR2	goqRT-G2-F	AATCTTCTCCACGTTTACCG
		goqRT-G2-R	CAGTAGAAGTAATTCATGGTG
	β-actin	goqRT-βactin-F	TCCCTGGAGAAGAGCTACGA
		goqRT-βactin-R	GTGTTGGCGTACAGGTCCTT

Degenerate bases: Y = C + T; W = A + T; and S = C + G.