Antioxidant and Proapoptotic Activities of Sclerocarya birrea [(A. Rich.) Hochst.] Methanolic Root Extract on the Hepatocellular Carcinoma Cell Line HepG2

Figure 3

Effect of MRE exposure on HepG2 cell morphology. HepG2 cells were treated with different concentrations of root extract for 24 h and morphological changes were observed using phase-contrast microscopy. The control cells show the normal morphology. In contrast, cells treated with intermediate concentrations of MRE show abundant cytoplasmic vacuoles. At high dosage of treatment, cells became round and shrunken. The photographs were taken at a magnification ×40. Images are representative of three independent experiments ((a)-(b) control; (c)-(d) 10 μg/mL; (e)-(f) 50 μg/mL; (g)-(h) 100 μg/mL; (i)-(j) 200 μg/mL; (b), (d), (f), (h), and (j) +10 mM NAC).

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