Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting

Figure 5

Change in glucan conversion plotted against change in xylan conversion for simultaneous hydrolysis of barley straw (a) alfalfa hay (c) by optimized enzyme mix and their respective controls ((b), (d); only rumen mix enzymes). Optimized enzyme mix composition used for barley straw and alfalfa hay digestion was identical to those used in Figure 3. Final enzymes load of 30 mg/g glucan with 2% solid load in 5 mL reaction volume was used.

(a)

(b)

(c)

(d)