Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting

Table 1

Sources of gene information, materials for production of recombinant enzymes, and properties of recombinant enzymes used in this study.

Clone ID	Entry name	Enzyme activity	CAZy family	Organism	mycoCLAP entry name	JGI ID	UniProt ID	Production host	pH optimum	Temp opt
Anig_Anig200605C	ABF54B_ASPNG	Alpha-N-arabinofuranosidase	GH54	Aspergillus niger N400	ABF54B_ASPNG	200605	P42255^a	Aspergillus niger py11	4.5	50
Anig_Anig214598C	PGA28A_ASPNG	Endopolygalacturonase	GH28	Aspergillus niger N400	PGA28A_ASPNG	214598	Q9P4W4^a	Aspergillus niger py11	4	60
Asn194096	AXE16B_ASPNG	Acetylesterase	CE16	Aspergillus niger N400	To be submitted	194096	G3Y7Z1^a	Aspergillus niger py11	5	60
Asn7870	AXE16A_ASPNG	Acetylesterase	CE16	Aspergillus niger N400	To be submitted	54865	G3Y497^a	Aspergillus niger py11	6	55
Anig_TterXXXX7G	EGL7A_THITE	Endoglucanase	GH7	Thielavia terrestris	To be submitted	54138	G2QZA7^b	Aspergillus niger py11	6	55
—	FAE 1a	Ferulic acid esterase	CE1	Anaeromyces mucronatus	—	—	F2YCB6^c	E. coli	7.2	37

The ID number corresponds to the protein ID of Aspergillus niger v3.0 annotation, http://genome.jgi-psf.org/Aspni5; ^bthe ID number corresponds to the protein ID of Thielavia terrestris v3.0 annotation, http://genome.jgi-psf.org/Thite2. ^cID for FAE 1a is a primary (citable) accession number (http://www.uniprot.org/uniprot/F2YCB6).