Research Article
Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting
Table 1
Sources of gene information, materials for production of recombinant enzymes, and properties of recombinant enzymes used in this study.
| Clone ID | Entry name | Enzyme activity | CAZy family | Organism | mycoCLAP entry name | JGI ID | UniProt ID | Production host | pH optimum | Temp opt |
| Anig_Anig200605C | ABF54B_ASPNG | Alpha-N-arabinofuranosidase | GH54 | Aspergillus niger
N400 | ABF54B_ASPNG | 200605 | P42255a | Aspergillus niger py11 | 4.5 | 50 |
| Anig_Anig214598C | PGA28A_ASPNG | Endopolygalacturonase | GH28 | Aspergillus niger N400 | PGA28A_ASPNG | 214598 | Q9P4W4a | Aspergillus niger py11 | 4 | 60 |
| Asn194096 | AXE16B_ASPNG | Acetylesterase | CE16 | Aspergillus niger N400 | To be submitted | 194096 | G3Y7Z1a | Aspergillus niger py11 | 5 | 60 |
| Asn7870 | AXE16A_ASPNG | Acetylesterase | CE16 | Aspergillus niger N400 | To be submitted | 54865 | G3Y497a | Aspergillus niger py11 | 6 | 55 |
| Anig_TterXXXX7G | EGL7A_THITE | Endoglucanase | GH7 | Thielavia terrestris | To be submitted | 54138 | G2QZA7b | Aspergillus niger py11 | 6 | 55 |
| — | FAE 1a | Ferulic acid esterase | CE1 | Anaeromyces mucronatus | — | — | F2YCB6c | E. coli | 7.2 | 37 |
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The ID number corresponds to the protein ID of Aspergillus niger v3.0 annotation, http://genome.jgi-psf.org/Aspni5; bthe ID number corresponds to the protein ID of Thielavia terrestris v3.0 annotation, http://genome.jgi-psf.org/Thite2. cID for FAE 1a is a primary (citable) accession number (http://www.uniprot.org/uniprot/F2YCB6).
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