Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 1

Comparison of HUVEC cultures at early and late passage. (a) Representative images of HUVEC cultures stained for the SA-β-gal (upper panels, dark grey cells) at passage 3 and passage 10. Lower panels show the digital images used to quantify the % SA-β-gal positive cells (red color; see materials and methods for details). Nuclei are stained with Hoechst (blue). Scale bars = 100 μm. Graph: percentage of SA-β-gal positive cells. for both analyses. test,   . Error bars indicate the standard error mean (s.e.m.). (b) Graphs show the expression levels of the P16 (left) and P21 (right) genes measured in HUVEC cultures. Values indicate the fold change (f.c.). All values were normalized to the expression levels of the housekeeping gene 18S. Error bars represent the s.e.m. For both analyses . P16 passage 3 = 1.1, passage 10 = 38.6. test:   ; P21 passage 3 = 1.0, passage 10 = 2.6. test:   .

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