Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 2

Acute vitamin E supplementation reduces the percentage of SA-β-gal positive HUVECs. (a) Representative images of the graph shown (b) comparing HUVEC cultures at passages 5 and 9 stained for the SA-β-gal (dark grey cells). Enlargements highlight the morphological differences observed comparing early and late passages. Nuclei are visualized in blue. Scale bars = 100 μm. (b) Graph showing the percentage of SA-β-gal positive cells measured in passage 5 and passage 9 HUVEC cultures. For all analyses . Passage 5: CTR = 22%, vitamin E = 16%. test   ; passage 9: CTR = 42%, vitamin E = 36%. test,   ; CTR (passage 5) versus CTR (passage 9) cultures: test,   . Error bars represent the s.e.m.

(a)

(b)