Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 5

Chronic vitamin E supplementation reduces the percentage of SA-β-gal positive and proliferating HUVECs. (a) Graph showing the percentage of SA-β-gal positive cells measured in HUVEC cultures at passages 5, 8, and 12. For all conditions . Error bars represent the s.e.m. Passage 5: CTR = 27%, vitamin E = 16%; test, ; passage 8: CTR = 37%, vitamin E = 32%; test, ; passage 12: CTR = 74%, vitamin E = 70%; test, . (b) Representative images of the graph shown in (a) comparing passage 5 and passage 12 HUVEC cultures stained for the SA-β-gal (dark grey cells). Enlargements highlight the morphological differences. Nuclei are visualized in blue (Hoechst staining). Scale bars = 100 μm. (c) Graph showing the percentage of EdU positive cells measured in HUVEC cultures at passages 5, 8, and 12. Error bars represent the s.e.m. For all analyses . Passage 5: CTR = 27.6%, vitamin E = 28.2%; test, (ns); passage 8: CTR = 22%, vitamin E = 20.7%; test, ; passage 12: CTR = 5.5%, vitamin E = 3.7%; test, .

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