Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 6

Chronic vitamin E supplementation reduces the expression levels of the P21 gene. P21 and P16 gene expression analysis in passage 5, 8, and 11 HUVEC cultures chronically treated with vitamin E. Error bars represent s.e.m. All values were normalized to the expression of the housekeeping gene 18S. Gene expression is indicated as f.c. Unless differently indicated, . (a) P21 gene expression. Passage 5: CTR = 1.0, vitamin E = 0.9; test: (ns). Passage 8: CTR = 1.1, vitamin E = 0.8; test, . Passage 11: CTR = 1.0; vitamin E = 0.5, ; test: . (b) P16 gene expression. Passage 5: CTR = 1.0, vitamin E = 1.1; test: (ns). Passage 8: CTR = 1.8, vitamin E = 1.9; test: (ns). Passage 11: CTR = 2.5; vitamin E = 2.0, ; test: (ns). CTR passage 5 versus passage 8 and passage 11: test, .

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