Quantitative Image Analysis of Epithelial and Stromal Area in Histological Sections of Colorectal Cancer: An Emerging Diagnostic Tool

Figure 4

β-catenin/K8 double staining in a colorectal cancer tissue section. (a) Merged image: β-catenin positive cells are visible in red, and K8 positive cells are shown in green. Nuclei are shown in light blue (all false colors). The same image is shown with individual fluorescence channels in grey scale: (b) K8, (c) β-catenin, and (d) nuclear staining with DAPI. Arrows with solid lines indicate cells with a translocation (cytoplasmic or nuclear) of β-catenin. Arrows with dashed lines indicate small groups of cells still positive for K8, but no more expressing β-catenin.

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