A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus

Figure 1

(a) Phylogenetic tree derived from the CP nucleotide sequences (572 nt) of Sugarcane streak mosaic virus (SCSMV). The tree was generated using MEGA 5 by the neighbor-joining method with 1,000 bootstrap replicates. The bootstrap confidence values are indicated when >60%. The NCBI accession number of the sequences used in the comparison is shown along with isolates’ name and the isolates from this study were marked with a black triangle. (b) Primers and probes sequences used in qRT-PCR assay aligned with the same set of CP sequences by DNAMAN version 8.

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