A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus

Figure 2

Standard curves for TaqMan probes-based real-time qRT-PCR using SCSMV CP transcripts (positive-sense single-stranded RNA, ssRNA) ((a) and (b)) and total RNA ((c) and (d)) of plant infected SCSMV YN-YZ211 isolate as templates. A tenfold dilution serial standard template containing ssRNA ranged from 1 × 10⁹ to 1 × 10² copies/reaction. Total RNA templates ranged from 2 pg to 200 ng/reaction. Ct values were measured in triplicate and plotted against the known copy numbers (log scale) or picograms of template total RNA (log scale). Two different probes, SCSMV-QPCR-P1 ((a), (c)) and SCSMV-QPCR-P2 ((b), (d)), were developed.

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(b)

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