A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus

Figure 3

Gel-based sensitivity tested using the two set primers of qRT-PCR (SCSMV-QPCR-F1 and -R1) ((a) and (c)) and conventional RT-PCR (SCSMV-CPF2 and -CPR2) ((b) and (d)). The same set of templates (10⁷–10² copies/reaction) of SCSMV CP transcripts (positive-sense single-stranded RNA, ssRNA) was used in (a) and (b), and the same set of diluted solutions (2 pg to 200 ng/reaction) of total RNA extraction of sugarcane leaf infected SCSMV YN-YZ211 isolate were carried out in (c) and (d). M1. DL 2,000 DNA marker; M2. 20 bp DNA ladder marker; NC. Virus-free total RNA (100 ng).

(a)

(b)

(c)

(d)