A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus

Table 1

List of the primers and probes used in this study.

Virus	Primer name	Sequence (5′-3′)	Fragment size (bp)
SCSMV	SCSMV-CPF2	TCATMTCTTCATCRGCCGC	572
	SCSMV-CPR2	ATCTTCYCTACGCAGGTCCG
	SCSMV-QPCR-F1	CGGGAAACCCATAATACCAC	115
	SCSMV-QPCR-R1	GTCGATTTCTGCTGGTGAGA
	SCSMV-QPCR-P1	FAM-TGCTGCATTGATTTCGTGATGGTG-TAMRA
	SCSMV-QPCR-P2	FAM-TGCTGCATTGATTTTGTGATGGTG-TAMRA
SCMV	SCMV-F4	GTTTTYCACCAAGCTGGAACAGTC	900
	SCMV-R3	AGCTGTGTGTCTCTCTGTATTCTC
SrMV	SrMV-F	ACAGCAGAWGCAACRGCACAAGC	850
	SrMV-R	CTCWCCGACATTCCCATCCAAGCC

SCMV-F4/SCMV-R3 and SrMV-F/SrMV-R published in Alegria et al. [22] and Xie et al. [20], respectively.
M = A/C, Y = C/T, W = A/T, R = A/G, and W = A/T in primer sequences.
FAM: 6-carboxyfluorescein, TAMRA: 6-carboxy tetramethyl rhodamine.