A One-Step Real-Time RT-PCR Assay for the Detection and Quantitation of Sugarcane Streak Mosaic Virus

Table 2

Detection of SCSMV from field sugarcane samples by real-time qRT-PCR with SCSMV-QPCR-P2 probe and conventional RT-PCR.

Varieties	Sampling location	qRT-PCR			Conventional RT-PCR
		Ct value	Copies/L	Result	SCSMV	SrMV	SCMV
FN38	Fuzhou, Fujian	30.53 ± 0.28	8.2 × 10^2	+	—	+, [KC984957]	—
FN15	Fuzhou, Fujian	na	na	−	—	+, [KC984956]	—
ROC22	Fuzhou, Fujian	30.37 ± 0.16	8.9 × 10^2	+	—	+, [KC984964]	+, [KC899320]
FN39	Zhangzhou, Fujian	na	na	−	—	+, [KC984958]	—
ROC22	Guangzhou, Guangdong	na	na	−	—	+, [KC984980]	—
F160	Suixi, Guangdong	20.51 ± 0.33	5.1 × 10^5	+	+, [KJ944725]	+, [KJ944744]	—
GT31	Suixi, Guangdong	33.51 ± 0.34	1.2 × 10^2	+	+, [KJ944729]	+, [KJ944745]	—
LC05-136	Suixi, Guangdong	na	na	−	—	+, [KJ944746]	—
ROC22	Suixi, Guangdong	32.68 ± 0.42	2.0 × 10^2	+	+, [KJ944727]	+, [KJ944741]	—
YZ05-49	Suixi, Guangdong	19.34 ± 0.14	1.1 × 10^6	+	+, [KJ944728]	+, [KJ944742]	—
YZ07-2384	Zhanjiang, Guangdong	16.72 ± 0.23	5.8 × 10^6	+	+, [KJ944726]	+, [KJ944736]	—
GT11	Wangmo, Guizhou	32.55 ± 0.30	2.2 × 10^2	+	+, [KC985070]	+, [KC985029]	—
GZ18	Wangmo, Guizhou	31.39 ± 0.5	4.6 × 10^2	+	+, [KC985071]	—	—
YZ03-45	Wangmo, Guizhou	32.21 ± 0.15	2.7 × 10^2	+	+, [KC985072]	+, [KC985030]	—
YG35	Kaiyuan, Yunnan	18.42 ± 0.36	1.9 × 10^6	+	+, [KC985085]	+, [KC985056]	—
YN01-58	Kaiyuan, Yunnan	31.26 ± 0.25	5.0 × 10^2	+	+, [KC985086]	+, [KC985061]	—
YZ05-211	Kaiyuan, Yunnan	21.19 ± 0.29	3.3 × 10^5	+	+, [KC985090]	+, [KC985064]	—
YT93-159	Lincang, Yunnan	32.59 ± 0.31	2.1 × 10^2	+	—	+, [KC985060]	—
FN15	Danzhou, Hainan	32.35 ± 0.22	2.5 × 10^2	+	—	+, [KC985031]	—
YT55	Danzhou, Hainan	31.52 ± 0.27	4.2 × 10^2	+	—	+, [KC985038]	—
ROC22	Haikou, Hainan	32.07 ± 0.07	3.0 × 10^2	+	+, [KC985074]	—	—
YZ05-49	Lingao, Hainan	16.44 ± 0.27	6.9 × 10^6	+	+, [KC985075]	+, [KC985041]	—
GT02-281	Beihai, Guangxi	32.13 ± 0.19	2.9 × 10^2	+	+, [KC985069]	+, [KC984999]	—
FN1110	Bingyang, Guangxi	na	na	−	—	+, [KJ944748]	—
YT93-159	Bingyang, Guangxi	na	na	−	—	+, [KJ944750]	+, [KJ944720]
FN39	Laibin, Guangxi	32.27 ± 0.32	2.6 × 10^2	+	+, [KJ944735]	+, [KJ944782]	—
ROC16	Laibin, Guangxi	32.17 ± 0.21	2.8 × 10^2	+	—	+, [KJ944757]	—
ROC22	Laibin, Guangxi	29.60 ± 0.12	1.5 × 10^3	+	+, [KJ944731]	+, [KJ944764]	—
YG46	Laibin, Guangxi	32.92 ± 0.35	1.7 × 10^2	+	+, [KJ944734]	+, [KJ944774]	—
YR06-189	Laibin, Guangxi	32.89 ± 0.46	1.8 × 10^2	+	—	+, [KJ944758]	—
GT05-3084	Longan, Guangxi,	na	na	−	—	+, [KJ944820]	—
GT07-994	Liuzhou, Guangxi	na	na	−	—	+, [KJ944814]	—
ROC22	Liuzhou, Guangxi	na	na	−	—	+, [KJ944815]	—
FN15	Shanglin, Guangxi	na	na	−	—	+, [KJ944752]	—
ROC22	Shanglin, Guangxi	na	na	−	—	+, [KJ944754]	—

Positive result (+) if Ct ≤ 35, negative result (−) if Ct > 35.
GenBank acc. no. of these sequences from conventional RT-PCR product were in brackets.
na: data not available.