Design and Synthesis of a Novel Cationic Peptide with Potent and Broad-Spectrum Antimicrobial Activity

Table 1

The sequences of six designed peptides.

Name	Sequence	Design
KWKK-13	KWKKPKLLKKLLK	N-terminal domain contains three cationic lysine residues and C-terminal is a standard α-helix (KLLK). They are connected with proline.
KW-13	KWKYPKLLKKLLK	N-terminal domain contains two cationic lysine residues and C-terminal is a standard α-helix (KLLK). They are connected with proline.
RFFR-15	RRWWRFPRFFRRFFR-NH2	N-terminal domain contains three cationic arginines and C-terminal is a standard α-helix (RFFR). They are connected with proline. C-terminal amidation stabilizes the peptide.
RFPP-18	RRWWRFPPPRFPPRFPPP-NH2	N-terminal domain contains three cationic arginines and C-terminal is a standard α-helix (RFPP). They are connected with proline. C-terminal amidation stabilizes the peptide. This sequence contains more proline.
KPV-13	KWKLFKKIWGKPV-NH2	Hybrid peptide based on the AMPs in the database. The N-terminal is from cecropin A1 and C-terminal is from MSH.
KPV-8	KFRWGKPV-NH2	Hybrid peptide based on the AMPs in the database. The N-terminal is from cecropin A1 and C-terminal is from MSH.