Attenuating Tumour Angiogenesis: A Preventive Role of Metformin against Breast Cancer

Figure 3

Effect of metformin treatment on tumour necrosis and angiogenesis. H&E staining of tumours obtained from Metformin and Control groups. Scale bar: 200 μm ((a), upper panels) and 100 μm ((a), lower panels). (b) Immunofluorescent staining of vWF (green) in tumours obtained from Metformin and Control groups. Scale bar: 100 μm. Nuclei were counter-stained with DAPI (blue). Area of tumour necrosis (c) and tumour blood vessel density (d) were quantified. Tumour necrosis was indicated by black arrows. Data are presented as means ± SD, -5. Images are representative of 4-5 animals from 3 separate experiments.

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