Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells

<div>Vitamin D3 inhibits transforming growth factor-<i>β</i>1- (TGF-<i>β</i>1-) induced <i>α</i>-smooth muscle actin (<i>α</i>-SMA) upregulation of mesothelial cells (MCs)<i> in vitro</i>. MCs were treated with TGF-<i>β</i>1 with or without addition of 1<i>α</i>,25(OH)<sub>2</sub>D<sub>3</sub> (vit. D). Immunofluorescence analysis showed that TGF-<i>β</i>1 promoted upregulation of <i>α</i>-SMA (green) in MCs and vitamin D inhibited this phenomenon. Nuclei are stained with DAPI (blue). This inhibition effect is quantified in (b) where the positive staining for <i>α</i>-SMA was calculated as a percentage of MCs present. Data represent mean ± S.D. 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BioMed Research International

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Figure 8

Figure 8: Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells 

Figure 8 | Vitamin D Can Ameliorate Chlorhexidine Gluconate-Induced Peritoneal Fibrosis and Functional Deterioration through the Inhibition of Epithelial-to-Mesenchymal Transition of Mesothelial Cells 