Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 2

LPA effects on apoptosis induced by cisplatin in Hela cell. (a) Cell pictures (×20). Hela cells were treated with cisplatin at different doses. After 8 hours of treatment with cisplatin, many floating cells were observed. The bigger the dose of cisplatin, the more the floating cells. (b) Flow cytometry detection of apoptosis with Annexin V/PI staining. Hela cells were treated with cisplatin at different doses for 4 hours. Apoptotic cells were detected and quantified with flow cytometry. Increased apoptosis following cisplatin treatment was observed. (c) Quantification of LPA effects on apoptosis induced by cisplatin. Hela cells were treated with cisplatin (at 200 μg/mL) and LPA (at different concentrations) for 4 hours. The apoptotic cells were detected by flow cytometry and the apoptosis rate of Hela cells was quantified and shown. Cell apoptosis induced by cisplatin was significantly inhibited by LPA. (d) Caspase-3 activity. Hela cells were treated with 200 μg/mL of cisplatin plus LPA at 20 μM for 4 hours. The caspase-3 activity was measured and shown. Cisplatin dramatically increased the caspase-3 activity, but the upregulated activity of caspase-3 was partially reversed by LPA treatment.

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