Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 5

Flow cytometry detection of cell apoptosis by Annexin V/PI staining. (a) Hela cells were treated with cisplatin (200 μg/mL) and LPA (20 μM), alone or in combination with different inhibitors for 4 hours. Cisplatin and LPA were used in each inhibitors-treated group. Inhibitors including PD98059 (MAPK inhibitor, at 10 μM), PD98059 (MAPK inhibitor, at 10 μM), and Y-27632 (Rho inhibitor, at 10 μM) were applied, respectively. (b) The apoptosis rate of Hela cells was quantified. Cisplatin-treated group served as control. PI3K inhibitor LY294002 significantly reversed the LPA effect on apoptosis induced by cisplatin, while MAPK inhibitor PD98059 and Rho inhibitor Y-27632 had no significant effect.

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