Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 7

Western blotting analysis of phosphorylated ERK in cells treated with LPA. (a) Hela cells were treated with LPA at different concentrations including 0, 0.1 μM, 1 μM, 5 μM, 20 μM, and 50 μM, for 1 hour. Western blotting was performed to detect the levels of phosphorylated ERK. Total ERK was used as protein input control. The density of bands was quantified and compared. LPA activated the phosphorylation of ERK in a dose-dependent manner. (b) Hela cells were treated with LPA at 20 μM for different times including 5 min, 10 min, 30 min, 1 hour, 2 hours, 3 hours, and 4 hours. A time-dependent increase in the ERK phosphorylation was detected.

(a)

(b)