Research Article
Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro
Table 1
Primers for expressed genes in WT and trk-gene knockout mutant strains of Mtb.
| | Gene name | Forward primer (bp) | Reverse primer (bp) | Fragment length | Tm |
| | CGG CGA CAA CTC CAA CAT (18) | CGG CAC ACC GAA GGT TTC (18) | 58 | 86.2 | | CTG CTG GAG TCG ATT CAC CT (20) | ACC GCG AAT TCG GTC TTG (20) | 107 | 88.9 |
| | kdpA | GAT TGA ACG GCC TAC TGG TC (20) | CTG GAT CTT CTT GCC GAG AT (20) | 95 | 87.5 | | kdpB | CTG GGC TGA CGA TCA TCT TT (20) | CGT GGT CGG AAT GAG ACA C (19) | 122 | 90.6 | | kdpC | TAC CGC AAG GAA AAC AAT CTG (21) | GGC ATT GAC CAC CGA TATG (19) | 102 | 90.2 | | kdpF | AAC ATC GTC GGG TTG GTG (18) | GCG AAT AGG AAC GCC ATT AG (20) | 50 | 83.7 |
| | kdpD | AGT CCA TCG ACC AAC TCA CC (20) | CAC CGC TTC CTC CAG GTA T (19) | 110 | 91.4 | | kdpE | TGG AAT GGA CGA GTT TCT GG (20) | GAC GGT GAA TGA ATC GGT TT (20) | 103 | 91.4 |
| | sigA | CAA GGA CGC CGA ACT CAC (18) | CTT GCC GAT CTG TTT GAG GT (20) | 64 | 87.4 |
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Size of the amplicon synthesized by RT-PCR in base pair (bp).
Melting temperature derived from melting curve.
Gene encoding Trk system.
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