Screening and Identification of ssDNA Aptamer for Human GP73

Figure 3

The binding capacity of A10-2 for GP73. (a) The recombinant protein GP73 was plated in a series of concentrations (31.25 ng/mL to 4 μg/mL) and incubated with Dig-labeled A10-2. Finally, anti-Dig-ALP antibodies were added and revealed with PNPP solution at 405 nm. (b) Three concentrations of recombinant protein GP73 were plated and incubated with several concentrations (0.078–40 ng/μL) of Dig-labeled A10-2. Afterwards, anti-Dig-ALP antibodies were added and revealed as above. The of A10-2 was calculated by nonlinear regression analysis. All experiments were performed in triplicate and repeated at least for three times.

(a)

(b)