Optimization of Culture Conditions for Production of the Anti-Leukemic Glutaminase Free L-Asparaginase by Newly Isolated Streptomyces olivaceus NEAE-119 Using Response Surface Methodology

Figure 3

The phylogenetic tree was constructed via the bootstrap test of neighbor-joining algorithm based on the 16S rRNA gene sequences of strain NEAE-119 and related species of the genus Streptomyces. Only bootstrap values above 50%, expressed as percentages of 1000 replications, are shown at the branch points. GenBank sequence accession numbers are indicated in parentheses after the strain names. Phylogenetic analyses were conducted in the software package MEGA4. Bar: 0.5 substitution per nucleotide position.