Application of AMOR in Craniofacial Rabbit Bone Bioengineering

Figure 2

Bone tissue healing is evaluated by histological and Histomorphometric analysis. Critical size 8 mm bone defects were created in rabbit calvaria, which were implanted with collagen sponge alone (NEG) or with immobilized antibodies and sacrificed at 6 weeks. The antibodies tested were isotype-matched control Ab (Iso), polyclonal Ab (pAb), or BMP-2-specific Ab clones (C3, C15, C18, C20, C22, C24, and 3G7). H&E (left panels) and Trichrome blue staining (right panels) were performed (a). Histomicrographs are presented in low and high magnifications (16x, 100x, and 400x). Newly formed bone is embedded with osteocytes (black arrows, a) and quantified by histomorphometry (b). Percentage of osteoid bone area (mm²) demonstrated significant bone deposition in the defect region when treated with C22 and 3G7 anti-BMP-2 mAb clones. Means and standard deviations of replicate groups are shown (Neg, ISO, C22, 3G7). ^* or ^**, versus isotype control.

(a)

(b)