Role of TLR4-Mediated PI3K/AKT/GSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes

Figure 1

The effect of LPS on BRL-3A cells. The control group was untreated, while LPS (10 μg/mL) was used to treat separate groups of BRL-3A cells. (a) Cell viability of BRL-3A cells at 0–24 h after LPS stimulation was detected by CCK-8 assay. (b) Cells were stained for FITC-Annexin V and Propidium Iodide and then sorted and analyzed quantitatively by flow cytometry. Cell apoptotic rates are reported in the histograms. (c) Hoechst 33342 fluorescence staining of BRL-3A cells at different time points after LPS stimulation. (A) Control group: intact BRL-3A cells. (B) Karyopyknosis (white arrow) 12 h after LPS stimulation. (C) Nuclear fragmentation (red arrow) and apoptotic body (green arrow) 24 h after stimulation. Scale bars = 10 μm. Data were mean ± SEM. and versus control group.

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