Helicobacteraceae in Bulk Tank Milk of Dairy Herds from Northern Italy

Table 1

Results of molecular and bacteriological analysis performed on 163 bulk tank milk samples.

Number of milk samples	PCR					Bacteriological analysis^b
	Helicobacteraceae		H. pylori^a	“Candidatus Helicobacter bovis”^a	Wolinella spp.^a
	16S rRNA	23S rRNA^a
156	−	nd	nd	nd	nd	−
3	+	+	−	−	−	−
2	−	+	nd	nd	nd	Arcobacter butzleri
1	−	+	nd	nd	nd	Arcobacter cryaerophilus
1	−	+	nd	nd	nd	Arcobacter skirrowii

PCRs for 23S rRNA gene of Helicobacter, H. pylori, “Candidatus Helicobacter bovis,” and Wolinella spp. were performed on samples positive for the screening PCR for 16S rRNA gene of Helicobacteraceae. ^bStrain identification was performed by 16S rRNA gene sequencing (nd: not done).