Research Article
The Osteogenic Properties of Multipotent Mesenchymal Stromal Cells in Cultures on TiO2 Sol-Gel-Derived Biomaterial
Figure 2
The results of phenotypic characterization of cells used for the test (a) and the results of their osteogenic and adipogenic differentiation (b). (a) Images show positive reaction with specific antibodies detecting CD29, CD44, CD73, and CD105, respectively. No specific reaction was noted with anti-CD45 antibodies. DAPI staining was used to show localization of nuclei, presented in the figures as white dots. Negative controls are presented to show specificity of performed staining. Scale bar = 50 μm. (b) Images present morphology of rat ASCs and BMSCs cultured in DMEM medium supplemented with 10% of FBS (standard culture) and results of its osteogenic and adipogenic stimulation. For visualization of calcium deposits specific Alizarin Red staining was used, while lipid drops were stained using Oil-Red O. Scale bar = 200 μm.
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