BRCA1 185delAG Mutation Enhances Interleukin-1β Expression in Ovarian Surface Epithelial Cells

Figure 1

IL-1β protein levels are increased in 185delAG BRCA1 mutation carriers. (a) Normal NFH and FH BRCA1 185delAG IOSE cells were analyzed for precursor (pro-) and cleaved IL-1β protein expression via western blot. Blots were then stripped and probed for β-actin as a loading control. Values represent relative densitometry. (b) Cells were plated in triplicate at similar densities and conditioned media were collected as described. IL-1β ELISA activity assay was performed in triplicate and the results are expressed as the mean ± standard error. Symbol () denotes statistical significance at the 0.04 confidence level.

(a)

(b)