BRCA1 185delAG Mutation Enhances Interleukin-1β Expression in Ovarian Surface Epithelial Cells

Figure 2

IL-1β protein and message levels are increased in stably transfected BRAT cells. (a) Cells stably expressing BRAT or the pcDNA3.1 cells were plated at equal densities and RNA was isolated, DNAse-treated, and reverse-transcribed. Real-time PCR was performed in triplicate for IL-1β and actin using SYBR Green detection and the data are expressed as the mean fold difference ± standard error. (b) pcDNA3.1 and BRATc1 cells were analyzed for precursor (pro-) and cleaved IL-1β protein expression via western blot. Blots were then stripped and probed for β-actin as a loading control. Values represent relative densitometry. (c) pcDNA3.1, BRATc1, and BRATc2 cells were plated in triplicate at similar densities and conditioned media were collected. IL-1β ELISA activity assay was performed in triplicate and the data are expressed as the mean ± standard error. Symbol () denotes statistical significance at the 0.01 confidence level between BRAT and PCDNA3.1 cells.

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