BRCA1 185delAG Mutation Enhances Interleukin-1β Expression in Ovarian Surface Epithelial Cells

Figure 4

BRAT alters levels of inflammasome constituents. (a) Cells stably expressing pcDNA3.1 or BRAT (BRATc1 and BRATc2) were analyzed for precursor (pro-caspase-1) and cleaved caspase-1 (caspase-1 p20) protein levels via western blot. Blots were then stripped and probed for β-actin as a loading control. Values represent relative densitometric units. (b) Stable pcDNA3.1 or BRATc1 cells were analyzed for ASC protein expression via western blot. Blots were then stripped and probed for β-actin as a loading control. Values represent relative densitometric units. (c) Lysates obtained from stable pcDNA3.1 and BRATc1 cells were immunoprecipitated with ASC antibody and then analyzed via western blot for ASC and NALP3. Values represent densitometric units.

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