Doxorubicin Differentially Induces Apoptosis, Expression of Mitochondrial Apoptosis-Related Genes, and Mitochondrial Potential in BCR-ABL1-Expressing Cells Sensitive and Resistant to Imatinib

Figure 3

Relative mRNA expression of mitochondrial cytochrome b (MT-CYB) and NADH dehydrogenase 3 (MT-ND3) genes determined by real-time PCR in mouse-derived 32D BCR-ABL1+ cells sensitive to imatinib (S) or cells with imatinib resistance caused by the Y253H mutation in the BCR-ABL1 gene (253) or acquired imatinib resistance (AR). The cells were exposed at 37°C for 24 h to doxorubicin (DOX) at 1.0 µM. Presented is a ratio of expression for DOX-exposed and DOX-nonexposed cells. The expression of either gene was normalized to the mouseβ-actin gene; n = 3 for each cell line; the numbers above bars indicate significant p values for comparison between pairs connected by brackets.