Activation of Sonic Hedgehog Leads to Survival Enhancement of Astrocytes via the GRP78-Dependent Pathway in Mice Infected with Angiostrongylus cantonensis

Figure 3

Induction of apoptosis in activated astrocytes by culturing with Angiostrongylus cantonensis soluble antigens. Astrocytes were treated with the 500 μg antigens for 2 h. Cell apoptosis was analyzed by FASCan flow cytometer (Annexin V−/PI−: normal cells, Annexin V+/PI−: early stage apoptotic cells, Annexin V+/PI+: late stage apoptotic cells, and Annexin V−/PI+: necrosis cells) (, ).