Biological Production of an Integrin αvβ3 Targeting Imaging Probe and Functional Verification

Figure 3

(a) Endogenous integrin expression using immunostaining in CHO and U87MG cells. (b) Confocal fluorescence microscopy images of cells with or without integrin αvβ3. U87MG-EGFP and CHO-EGFP cells were incubated with 2 μg of GCR or GC protein at 37^°C for 1 h. Confocal microscopy imaging following treatment with the GCR protein revealed fluorescent signals from U87MG-EGFP cells but no signals from CHO-EGFP cells. Confocal microscopy following treatment with the GC protein revealed no fluorescent signals from U87MG-EGFP or CHO-EGFP cells. Scale bar corresponds to 10 μm. U87MG, human glioblastoma-astrocytoma, epithelial-like cells; CHO, Chinese hamster ovary cells; EGFP, enhanced green fluorescent protein.

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