Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs

Figure 1

Pareto chart of standardized effects on sublancin 168 production. , , , , , , , , , and are the coded values of peptone, corn powder, starch, soybean meal, KH₂PO₄, (NH₄)₂SO_4, incubation temperature, initial pH, incubation time, and inoculum size, respectively. The chart has a vertical line (i.e., standardized effect = 1.886) at the critical -value for α of 0.20. The bars are shown in order of the size of the effects, and the standardized effect of every term was displayed on the top of its corresponding bar.