Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs

Table 4

Experimental design and corresponding response of steepest ascent.

Experiment number	Corn powder (g/L)	Soybean meal (g/L)	Incubation temperature (°C)	Yield (mg/L)
0	12	8	25	72.7 ± 2.97
0 + 1Δ	16	12	27	80.3 ± 4.11
0 + 2Δ	20	16	29	89.5 ± 2.42
0 + 3Δ	24	20	31	117.5 ± 3.58
0 + 4Δ	28	24	33	122.6 ± 1.64
0 + 5Δ	32	28	35	109.0 ± 4.17

Each experiment was repeated three times, and all of the data were expressed as means ± standard deviations.