The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides

Figure 1

Representative structures of the CPP-DsRed and CPP-J-DsRed. Oligonucleotide pairs encoding DPV3A, E162, pVEC, R11, and TP13 listed in Table 1 were inserted between NdeI and EcoRI sites of pET22b to create pET22b-DPV3A, pET22b-E162, pET22b-pVEC, pET22b-R11, pET22b-PTD, and pET22b-TP13, respectively. The DsRed cDNA fragment cut from pET22b-PTD-J-DsRed by the EcoRI site at 5′-end and the XhoI site at 3′-end was inserted into the above vectors to prepare pET22b-DPV3A-DsRed, pET22b-E162-DsRed, pET22b-pVEC-DsRed, pET22b-R11-DsRed, pET22b-PTD-DsRed, and pET22b-TP13-DsRed. The J-DsRed cDNA fragment cut from pET22b-PTD-J-DsRed by the BamHI site at 5′-end and the XhoI site at 3′-end was inserted into the above vectors to prepare pET22b-DPV3A-J-DsRed, pET22b-E162-J-DsRed, pET22b-pVEC-J-DsRed, pET22b-R11-J-DsRed, and pET22b-TP13-J-DsRed.